Chromium next gem single cell 3 reagent kits v3 1

This page contains information on the available sequencing platforms and 10X Genomics reagents in order to help you estimate experiment scale and cost. In addition, please follow the instructions for ordering sequencing reagents and submitting samples when taking advantage of the DLMP sequencers.

Method Literature and Product Information

Current best practices for studying scRNA-Seq data is reviewed in the following article: Luecken MD and Theis FJ. Current foremost practices in single-cell RNA-seq analysis: A tutorial. Mol Syst Biol 15(6):e8746, 2019.

Further information on specific 10X Genomics assays and software be found in the following links:

1. Chromium Next GEM Unpartnered Cell 3’ Reagent Kits v3.1
2. Chromium Next GEM Single Cell V(D)J Reagent Kits v1.1
3. Chromium Next GEM Single Cell ATAC Reagent Kits v1.1
4. Chromium Single Cell Multiome ATAC + Gene Expression
5. 10X Genomics Cell Ranger data analysis software
6. 10X Genomics Loupe Browser data visualization software.

Further information on Duplex-Seq and akin technologies can be found in the following publications:

Original Article: Schmitt et al. Detection of ultra-rar

10x genomics chromium next gem single cell 3 reagent kits v3 1  (10X Genomics)

Journal: Cell reports

Article Title: Widespread sex-dimorphism across single-cell transcriptomes of adult African turquoise killifish tissues

doi: 10.1016/j.celrep.2023.113237

Figure Lengend Snippet: (A) Schematic for tissue dissociation and sequencing. Blood, kidneys, livers, and spleens were isolated from 3 cohorts of 3–4 fish. Solid tissues were dissociated into single-cell suspensions. Single-cell RNA-seq libraries were made using the 10x Genomics protocol. (B-F) UMAP plots of the killifish tissue atlas denoted by (B) annotated cell type, (C) tissue of origin, (D) sex, (E) cohort, and (F) RNA velocity. (G) Expression UMAP for canonical marker genes for B-cells, macrophages, neutrophils, and hepatocytes. (H) Dotplot of marker gene expression, showing average expression level and percentage of cells expressing each marker gene. Markers of cell proliferation (pcna and mki67) are provided under a yellow overlay, since they were used to annotated proliferative states. Since many NCBI gene names were uninformative, names corresponding to predicted homologs are used. O

Chromium Next GEM Single Cell 3' HT Reagent Kits v3.1
99Pages

Catalog excerpts

Chromium Next GEM Single Cell 3' HT Reagent Kits v3.1 (Dual Index) with Feature Barcode technology for Cell Surface Protein For use with: Chromium Next GEM Single Cell 3' HT Kit v3.1 48 rxns PN-1000348 | 8 rxns PN-1000370 Chromium Next GEM Chip M Single Cell Kit* 80 rxns PN-1000349 | 16 rxns PN-1000371 (*Included with Chromium Next GEM Single Cell 3' HT Kit v3.1; 16 rxn kit can also be ordered separately) 3' Feature Barcode Kit 16 rxns PN-1000262 Dual Index Kit TT Set A 96 rxns PN-1000215 Dual Index Kit NT Set A 96 rxns PN-1000242

Open the catalog to page 1

User Guide | Chromium Next GEM Single Cell 3' HT Reagent Kits v3.1 (Dual Index) Notices Document Number CG000417 | Rev D Legal Notices © 2022 10x Genomics, Inc. (10x Genomics). All rights reserved. Duplication and/or reproduction of all or any portion of this document without the express written consent of 10x Genomics, is strictly forbidden. Nothing contained herein shall constitute any warranty, express or implied, as to the performance of anyproducts described herein. Any and all warranties applicable to any product

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